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. 2017 Dec 15;130(24):4155–4167. doi: 10.1242/jcs.211102

Fig. 1.

Fig. 1.

Transport factors are upregulated in response to TSH stimulation. (A) Immunofluorescence staining of the indicated transport factors performed on FRTL-5 cells grown under basal (−TSH) conditions or cells treated with TSH (+TSH, 1 mIU/ml, 24 h). NIS was used as positive control for induction. Images are representative of three independent experiments. Scale bar: 10 μm. (B) Western blot analysis of lysates from FRTL-5 cells incubated under basal (−TSH) or stimulated (+TSH, 1 mlU/ml, 24 h) conditions. NIS and GAPDH were used as controls for TSH induction and loading, respectively. (C) Densitometric quantification of proteins shown in B normalized to GAPDH. Values represent fold change relative to protein levels under basal conditions (see Materials and Methods). Bar graph represents mean±s.e.m. of at least three independent experiments (•P<0.05; ••P<0.01). (D) Quantification of transport factors mRNA by qRT-PCR from total RNA obtained from FRTL-5 cells grown under basal (−TSH) or stimulated (+TSH, 1 mlU/ml, 14 h) conditions. NIS was used as positive control of TSH induction. Results were normalized to the levels of β-actin and expressed according to the 2−ΔΔCt method relative to the expression level in basal conditions (set as 1). Results are mean±s.e.m. of at least three independent experiments performed in triplicate (•••P<0.001).