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. 2017 Dec 15;130(24):4155–4167. doi: 10.1242/jcs.211102

Fig. 2.

Fig. 2.

Cargo proteins and transport factors display similar kinetics of expression in response to TSH stimulation. (A) Western blot of lysates from FRTL-5 cells stimulated with TSH (1 mIU/ml) for the indicated times. NIS was used as positive control for TSH induction, and GAPDH as a loading control. (B) Densitometric quantification of proteins in A normalized to GAPDH. Values represent fold change relative to protein levels at 0 h (see Materials and Methods). Results are mean±s.e.m. of at least three independent experiments. nd, not detectable (•P<0.05; ••P<0.01; •••P<0.001). (C) Quantification of mRNA levels by qRT-PCR with total RNA obtained from FRTL-5 cells stimulated with TSH (1 mIU/ml) for the indicated times. Results were normalized to β-actin and expressed according to the 2−ΔΔCt method relative to the expression level at 0 h (set as 1). Results are mean±s.e.m. of three independent experiments performed in triplicate (•P<0.05; ••P<0.01; •••P<0.001).