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. 2017 Dec 15;130(24):4155–4167. doi: 10.1242/jcs.211102

Fig. 5.

Fig. 5.

TSH stimulates CREB3L1 expression. (A) Western blot with antibodies against the N-terminal region of CREB3L1 or CREB3L2 in lysates from FRTL-5 cells stimulated with TSH (1 mIU/ml) for the indicated times. GAPDH was used as the loading control. (B) Densitometric quantification of proteins in A normalized to the levels of GAPDH. Values are the fold change relative to levels in cells at 0 h. Results are mean±s.e.m. of three independent experiments (•P<0.05; ••P<0.01). (C) Quantification of CREB3L1 and CREB3L2 mRNA levels by qRT-PCR performed with total RNA from FRTL-5 cells stimulated with TSH (1 mlU/ml) for the indicated times. Results are normalized to the levels of β-actin, expressed according to the 2−ΔΔCt method relative to levels at 0 h (set as 1) and are shown as mean±s.e.m. of three independent experiments performed in triplicate (•••P<0.001).