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. 2019 May 8;10:289. doi: 10.3389/fendo.2019.00289

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Copyright © 2019 Fuentes-García, Castañeda-Patlán, Vercoutter-Edouart, Lefebvre and Robles-Flores.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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O-GlcNAcylation levels and OGT expression are higher in colon cancer cell lines compared with normal colon cell line while OGA expression diminished in colon malignant cells compared with non-malignant ones. (A) Flow cytometry. The data represent the means ± SEM from at least three independent assays ^*p = 0.01; ^***p = 0.001; ^****p = 0.0001 compared with control 112CoN cells. (B) Western blot and (C) Immunofluoresce assay showing the different levels of O-GlcNAcylation and OGT expression in each cell line. Normal cells: 112CoN, cancer cells: SW480 and SW620.