Table 1.
Ratio Analysis for Paired Antibodies
| Antibody list | Signal ratio |
Fold change |
||
|---|---|---|---|---|
| without glucose | with glucose | with/without glucose | ||
| 14-3-3 zeta (Phospho-Ser58) | 14-3-3 zeta (Ab-58) | 1.92 | 1.88 | 0.98 |
| 4E-BP1 (Phospho-Thr36) | 4E-BP1 (Ab-36) | 1.57 | 1.54 | 0.98 |
| Akt (Phospho-Thr308) | Akt (Ab-308) | 0.77 | 0.84 | 1.09 |
| Akt (Phospho-Ser473) | Akt (Ab-473) | 0.74 | 0.91 | 1.22 |
| Akt2 (Phospho-Ser474) | Akt2 (Ab-474) | 1.17 | 1.18 | 1.01 |
| AMPK1 (Phospho-Thr174) | AMPK1 (Ab-174) | 1.76 | 1.56 | 0.89 |
| BAD (Phospho-Ser112) | BAD (Ab-112) | 0.86 | 0.98 | 1.15 |
| BAD (Phospho-Ser136) | BAD (Ab-136) | 1.00 | 1.10 | 1.10 |
| BAD (Phospho-Ser155) | BAD (Ab-155) | 1.04 | 1.16 | 1.12 |
| BCL-2 (Phospho-Thr56) | BCL-2 (Ab-56) | 0.85 | 0.91 | 1.07 |
| BCL-2 (Phospho-Ser70) | BCL-2 (Ab-70) | 0.66 | 0.72 | 1.09 |
| BCL-XL (Phospho-Ser62) | BCL-XL (Ab-62) | 0.09 | 0.09 | 1.05 |
| Beta-Catenin (Phospho-Ser37) | Beta-Catenin (Ab-37) | 1.12 | 1.21 | 1.08 |
| Beta-Catenin (Phospho-Thr41/Ser45) | Beta-Catenin (Ab-41/45) | 2.10 | 1.99 | 0.95 |
| BRCA1 (Phospho-Ser1423) | BRCA1 (Ab-1423) | 0.14 | 0.15 | 1.05 |
| BRCA1 (Phospho-Ser1524) | BRCA1 (Ab-1524) | 0.88 | 0.91 | 1.04 |
| CaMKII (Phospho-Thr286) | CaMKII (Ab-286) | 0.84 | 0.96 | 1.14 |
| Caspase 3 (Phospho-Ser150) | Caspase 3 (Ab-150) | 1.12 | 1.16 | 1.03 |
| Caspase 9 (Phospho-Thr125) | Caspase 9 (Ab-125) | 0.61 | 0.65 | 1.06 |
| Caspase 9 (Phospho-Ser144) | Caspase 9 (Ab-144) | 0.80 | 0.79 | 0.99 |
| Caspase 9 (Phospho-Tyr153) | Caspase 9 (Ab-153) | 0.88 | 1.02 | 1.17 |
| Caspase 9 (Phospho-Ser196) | Caspase 9 (Ab-196) | 0.40 | 0.37 | 0.93 |
| Caveol in-1 (Phospho-Tyr14) | Caveol in-1 (Ab-14) | 1.81 | 1.91 | 1.06 |
| CDC2 (Phospho-Tyr15) | CDC2 (Ab-15) | 0.72 | 0.96 | 1.32 |
| cdc25A (Phospho-Ser75) | cdc25A (Ab-75) | 1.36 | 1.56 | 1.15 |
| cdc25C (Phospho-Ser216) | cdc25C (Ab-216) | 0.43 | 0.45 | 1.05 |
| CDK2 (Phospho-Thr160) | CDK2 (Ab-160) | 0.87 | 1.00 | 1.16 |
| Chk1 (Phospho-Ser280) | Chk1 (Ab-280) | 1.28 | 1.41 | 1.10 |
| Chk1 (Phospho-Ser317) | Chk1 (Ab-317) | 0.82 | 0.85 | 1.04 |
| Chk1 (Phospho-Ser345) | Chk1 (Ab-345) | 1.19 | 1.19 | 1.00 |
| Chk2 (Phospho-Ser516) | Chk2 (Ab-516) | 0.97 | 1.15 | 1.19 |
| Chk2 (Phospho-Thr68) | Chk2 (Ab-68) | 0.35 | 0.39 | 1.11 |
| c-Jun (Phospho-Thr239) | c-Jun (Ab-239) | 1.04 | 1.10 | 1.05 |
| c-Jun (Phospho-Ser243) | c-Jun (Ab-243) | 0.45 | 0.51 | 1.14 |
| c-Jun (Phospho-Ser73) | c-Jun (Ab-73) | 0.20 | 0.19 | 0.96 |
| c-Kit (Phospho-Tyr721) | c-Kit (Ab-721) | 0.70 | 0.80 | 1.15 |
| CREB (Phospho-Ser133) | CREB (Ab-133) | 0.64 | 0.76 | 1.18 |
| CrkII (Phospho-Tyr221) | CrkII (Ab-221) | 0.61 | 0.62 | 1.01 |
| eEF2K (Phospho-Ser366) | eEF2K (Ab-366) | 0.67 | 0.67 | 1.01 |
| EGFR (Phospho-Tyr1110) | EGFR (Ab-1110) | 0.70 | 0.86 | 1.23 |
| eIF2a (Phospho-Ser51) | eIF2a (Ab-51) | 0.88 | 0.86 | 0.98 |
| elF4E (Phospho-Ser209) | elF4E (Ab-209) | 0.78 | 0.95 | 1.21 |
| Elk-1 (Phospho-Ser383) | Elk-1 (Ab-383) | 1.09 | 1.01 | 0.93 |
| ERK3 (Phospho-Ser189) | ERK3 (Ab-189) | 1.18 | 1.19 | 1.00 |
| ERK8 (Phospho-Thr175/Tyr177) | ERK8 (Ab-175/177) | 0.57 | 0.58 | 1.02 |
| Estrogen Receptor-alpha (Phospho-Ser167) | Estrogen Receptor-alpha (Ab-167) | 1.14 | 1.13 | 0.99 |
| FAK (Phospho-Tyr397) | FAK (Ab-397) | 1.14 | 1.05 | 0.92 |
| FAK (Phospho-Tyr861) | FAK (Ab-861) | 0.75 | 0.79 | 1.06 |
| FAK (Phospho-Tyr925) | FAK (Ab-925) | 1.03 | 1.23 | 1.20 |
| FGF Receptor 1 (Phospho-Tyr154) | FGF Receptor 1 (Ab-154) | 0.72 | 0.89 | 1.23 |
| FKHR (Phospho-Ser256) | FKHR (Ab-256) | 1.23 | 1.23 | 1.00 |
| GSK3 alpha (Phospho-Ser21) | GSK3 alpha (Ab-21) | 0.99 | 0.92 | 0.93 |
| GSK3 beta (Phospho-Ser9) | GSK3 beta (Ab-9) | 0.66 | 0.71 | 1.08 |
| HDAC8 (Phospho-Ser39) | HDAC8 (Ab-39) | 1.06 | 1.10 | 1.04 |
| HER2 (Phospho-Tyr877) | HER2 (Ab-877) | 1.12 | 1.17 | 1.04 |
| Histone H2A.X (Phospho-Ser139) | Histone H2A.X (Ab-139) | 0.84 | 0.88 | 1.05 |
| HSF1 (Phospho-Ser303) | HSF1 (Ab-303) | 0.75 | 1.05 | 1.40 |
| HSP27 (Phospho-Ser15) | HSP27 (Ab-15) | 1.61 | 1.64 | 1.02 |
| HSP27 (Phospho-Ser78) | HSP27 (Ab-78) | 0.96 | 1.23 | 1.28 |
| HSP90B (Phospho-Ser254) | HSP90B (Ab-254) | 1.17 | 1.48 | 1.26 |
| ICAM-1 (Phospho-Tyr512) | ICAM-1 (Ab-512) | 1.89 | 1.86 | 0.99 |
| IGF-1R (Phospho-Tyr1161) | IGF-1R (Ab-1161) | 1.29 | 1.31 | 1.01 |
| IkB-alpha (Phospho-Ser32/Ser36) | IkB-alpha (Ab-32/36) | 5.36 | 6.36 | 1.19 |
| IkB-alpha (Phospho-Tyr42) | IkB-alpha (Ab-42) | 1.76 | 1.88 | 1.06 |
| IkB-epsi lon (Phospho-Ser22) | IkB-epsi lon (Ab-22) | 0.48 | 0.47 | 0.96 |
| IKK alpha (Phospho-Thr23) | IKK alpha (Ab-23) | 0.64 | 0.77 | 1.21 |
| Integrin beta-3 (Phospho-Tyr773) | Integrin beta-3 (Ab-773) | 0.57 | 0.64 | 1.11 |
| Integrin beta-3 (Phospho-Tyr785) | Integrin beta-3 (Ab-785) | 1.11 | 1.10 | 0.99 |
| JAK1 (Phospho-Tyr1022) | JAK1 (Ab-1022) | 0.95 | 1.28 | 1.35 |
| JAK2 (Phospho-Tyr1007) | JAK2 (Ab-1007) | 0.65 | 0.73 | 1.13 |
| JAK2 (Phospho-Tyr221) | JAK2 (Ab-221) | 0.90 | 1.27 | 1.41 |
| JunB (Phospho-Ser259) | JunB (Ab-259) | 1.75 | 1.77 | 1.01 |
| JunB (Phospho-Ser79) | JunB (Ab-79) | 2.08 | 1.87 | 0.90 |
| JunD (Phospho-Ser255) | JunD (Ab-255) | 1.68 | 1.84 | 1.09 |
| Keratin 18 (Phospho-Ser33) | Keratin 18 (Ab-33) | 2.04 | 2.28 | 1.12 |
| MDM2 (Phospho-Ser166) | MDM2 (Ab-166) | 0.06 | 0.07 | 1.06 |
| MEK1 (Phospho-Ser217) | MEK1 (Ab-217) | 1.91 | 1.95 | 1.02 |
| MEK1 (Phospho-Ser221) | MEK1 (Ab-221) | 0.96 | 1.14 | 1.19 |
| MEK1 (Phospho-Thr291) | MEK1 (Ab-291) | 0.61 | 0.68 | 1.10 |
| MEK2 (Phospho-Thr394) | MEK2 (Ab-394) | 0.98 | 0.97 | 0.98 |
| Met (Phospho-Tyr1349) | Met (Ab-1349) | 0.73 | 0.84 | 1.15 |
| MKK3 (Phospho-Ser189) | MKK3 (Ab-189) | 0.84 | 0.98 | 1.16 |
| MSK1 (Phospho-Ser376) | MSK1 (Ab-376) | 1.82 | 1.97 | 1.08 |
| mTOR (Phospho-Ser2448) | mTOR (Ab-2448) | 1.91 | 1.99 | 1.04 |
| Myc (Phospho-Thr358) | Myc (Ab-358) | 0.24 | 0.28 | 1.17 |
| Myc (Phospho-Ser373) | Myc (Ab-373) | 1.57 | 1.58 | 1.00 |
| Myc (Phospho-Thr58) | Myc (Ab-58) | 1.41 | 1.42 | 1.00 |
| Myc (Phospho-Ser62) | Myc (Ab-62) | 1.73 | 1.57 | 0.91 |
| NFkB-p100/p52 (Phospho-Ser865) | NFkB-p100/p52 (Ab-865) | 0.44 | 0.41 | 0.93 |
| NFkB-p100/p52 (Phospho-Ser869) | NFkB-p100/p52 (Ab-869) | 1.02 | 1.04 | 1.02 |
| NFkB-p105/p50 (Phospho-Ser893) | NFkB-p105/p50 (Ab-893) | 0.88 | 0.93 | 1.06 |
| NFkB-p105/p50 (Phospho-Ser907) | NFkB-p105/p50 (Ab-907) | 0.94 | 0.98 | 1.04 |
| NFkB-p65 (Phospho-Thr254) | NFkB-p65 (Ab-254) | 0.90 | 0.83 | 0.92 |
| NFkB-p65 (Phospho-Ser529) | NFkB-p65 (Ab-529) | 1.53 | 1.61 | 1.05 |
| p21Cip1 (Phospho-Thr145) | p21Cip1 (Ab-145) | 1.19 | 1.25 | 1.06 |
| p27Kip1 (Phospho-Ser10) | p27Kip1 (Ab-10) | 1.39 | 1.42 | 1.02 |
| p27Kip1 (Phospho-Thr187) | p27Kip1 (Ab-187) | 1.25 | 1.15 | 0.92 |
| P38 MAPK (Phospho-Tyr182) | P38 MAPK (Ab-182) | 0.36 | 0.45 | 1.26 |
| p44/42 MAP Kinase (Phospho-Thr202) | p44/42 MAP Kinase (Ab-202) | 0.87 | 1.01 | 1.17 |
| p44/42 MAP Kinase (Phospho-Tyr204) | p44/42 MAP Kinase (Ab-204) | 0.96 | 1.21 | 1.26 |
| p53 (Phospho-Ser315) | p53 (Ab-315) | 0.39 | 0.53 | 1.35 |
| p53 (Phospho-Ser6) | p53 (Ab-6) | 1.30 | 1.42 | 1.09 |
| p70 S6 Kinase (Phospho-Ser424) | p70 S6 Kinase (Ab-424) | 1.62 | 1.41 | 0.87 |
| PDGF Receptor beta (Phospho-Tyr751) | PDGF Receptor beta (Ab-751) | 0.52 | 0.59 | 1.14 |
| PDK1 (Phospho-Ser241) | PDK1 (Ab-241) | 0.52 | 0.36 | 0.70 |
| PI3-kinase p85-alpha (Phospho-Tyr607) | PI3-kinase p85-alpha (Ab-607) | 0.68 | 1.01 | 1.47 |
| PI3-kinase p85-subunit alpha/gamma (Phospho-Tyr467/Tyr199) | PI3-kinase p85-subunit alpha/gamma (Ab-467/199) | 0.98 | 0.06 | 1.08 |
| PTEN (Phospho-Ser380/Thr382/Thr383) | PTEN (Ab-380/382/383) | 0.66 | 0.73 | 1.11 |
| Pyk2 (Phospho-Tyr402) | Pyk2 (Ab-402) | 1.48 | 1.58 | 1.07 |
| Rac1/cdc42 (Phospho-Ser71) | Rac1/cdc42 (Ab-71) | 1.83 | 1.79 | 0.98 |
| Raf1 (Phospho-Ser259) | Raf1 (Ab-259) | 1.01 | 1.02 | 1.01 |
| Rb (Phospho-Ser780) | Rb (Ab-780) | 0.93 | 1.07 | 1.15 |
| Rel (Phospho-Ser503) | Rel (Ab-503) | 1.15 | 1.25 | 1.08 |
| SAPK/JNK (Phospho-Thr183) | SAPK/JNK (Ab-183) | 0.77 | 1.08 | 1.40 |
| Shc (Phospho-Tyr349) | Shc (Ab-349) | 0.62 | 0.74 | 1.20 |
| SHP-2 (Phospho-Tyr580) | SHP-2 (Ab-580) | 1.93 | 2.04 | 1.05 |
| Src (Phospho-Tyr418) | Src (Ab-418) | 0.23 | 0.26 | 1.11 |
| Src (Phospho-Tyr529) | Src (Ab-529) | 1.52 | 1.48 | 0.98 |
| STAT1 (Phospho-Tyr701) | STAT1 (Ab-701) | 1.72 | 1.74 | 1.01 |
| STAT1 (Phospho-Ser727) | STAT1 (Ab-727) | 1.78 | 2.11 | 1.19 |
| STAT3 (Phospho-Tyr705) | STAT3 (Ab-705) | 1.18 | 1.16 | 0.98 |
| STAT3 (Phospho-Ser727) | STAT3 (Ab-727) | 1.56 | 1.54 | 0.99 |
| STAT4 (Phospho-Tyr693) | STAT4 (Ab-693) | 1.71 | 1.71 | 1.00 |
| STAT5A (Phospho-Tyr694) | STAT5A (Ab-694) | 1.62 | 1.46 | 0.90 |
| STAT5A (Phospho-Ser780) | STAT5A (Ab-780) | 0.15 | 0.16 | 1.08 |
| STAT6 (Phospho-Tyr641) | STAT6 (Ab-641) | 1.40 | 1.61 | 1.15 |
| STAT6 (Phospho-Thr645) | STAT6 (Ab-645) | 0.22 | 0.24 | 1.13 |
| Tau (Phospho-Ser404) | Tau (Ab-404) | 1.13 | 1.18 | 1.05 |
| Trk B (Phospho-Tyr515) | Trk B (Ab-515) | 0.32 | 0.37 | 1.13 |
| TYK2 (Phospho-Tyr1054) | TYK2 (Ab-1054) | 0.84 | 0.87 | 1.04 |
| VEGFR2 (Phospho-Tyr951) | VEGFR2 (Ab-951) | 0.19 | 0.21 | 1.08 |