Fig. 1.

MMG8 is a novel cis-Golgi protein. (A) HeLa extracts resolved using SDS-PAGE (6% and 15% gels) were probed with an anti-MMG8 antibody (443M) or the preimmune serum (PS). (B) HeLa extracts prepared in RIPA buffer were used for immunoprecipitation (IP). The immunoprecipitates were resolved using SDS-PAGE and immunoblotted with anti-MMG8 or stained with Coomassie Blue. The protein of ∼150 kDa was excised for analysis through mass spectrometry. (C) A schematic representation of the MMG variants. Identical sequence regions are shown in the same color. P1–P13 denote peptides analyzed using mass spectrometry. (D) Representative micrographs (100% of 100 cells) of HeLa cells stained for MMG8 and Golgi proteins. Boxed areas are enlarged on the right. Scale bars: 5 µm.