Fig. 7.
Effect of α-integrin CT truncation on kindlin-induced integrin conformational change. (A,B) Binding of LIBS mAb 319.4 and 370.3 to αIIbβ3 induced by the overexpression of EGFP–TH plus mCherry (mC)-tagged kindlin-2 (mC–K2) or kindlin-3 (mC–K3). (C,D) Binding of LIBS mAb KIM127 and M24 to αLβ2 induced by the overexpression of EGFP–TH plus mC–K2 or mC–K3. 293FT cells were co-transfected with the integrin constructs (shown under B and D) and EGFP–TH plus mC, mC–K2, or mC–K3. The integrin, EGFP, and mCherry triple-positive cells were analyzed by flow cytometry. The binding of LIBS mAb was presented as the MFI normalized to integrin expression. Data are presented as mean±s.e.m (n≥3). *P<0.05; **P<0.01; ***P<0.001; ns, not significant (unpaired two-tailed t-tests were used for the comparison with mC control group).