Fig. 8.

TGFB1 increases the permeability of CLENDO cell monolayers. CLENDO cells were grown to confluence on membrane inserts (3 μm pore). Cells were pretreated with or without SB-431542 (SB, 1 μM) and then treated with control medium (CTL) or TGFB1 (1 ng/ml) for 24 hours. (A) Monolayer permeability was measured by determining the fluorescence of the bottom chamber medium at the indicated times after the addition of the fluorescent tracer molecule FITC–dextran to the top chamber. Data represent mean ± s.e.m. of fluorescence from a representative experiment performed in triplicate. (B) At the end of the assay, monolayers were fixed and stained with crystal violet to show equivalent density of CLENDO monolayers.