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Principles of the conventional (A) and the mismatch-tolerant LAMP (B) methods. The presence of a mismatch at 3′-end of the primer will largely reduce or stop LAMP amplification. The presence of a small amount of high-fidelity DNA polymerase, which has 3′–5′ exonuclease activity, can remove the mismatched bases from the primers, allowing the Bst DNA polymerase to initiate primer extension.
