Figure 2.
Flexibility of the novel mismatch-tolerant RT-LAMP to various mismatches. (A) Primer and template sets. (B) Amplification curves of the novel mismatch-tolerant and the conventional RT-LAMP methods for various mutants that form mismatches with the primers. About 30,000 RNA copies of mutant or wild-type template were added in each reaction. WT, wild-type; Mu, mutant; NTC, no template control.