Table 1.

The influence of various mismatches between primers and templates on the amplification times by the novel mismatch-tolerant and the conventional RT-LAMP methods.

Template	The novel mismatch-tolerant RT-LAMP						The conventional RT-LAMP						Tt Diff. (Novel-Conv.)
	Tt-T1 (min)	Tt-T2 (min)	Tt-T3 (min)	Mean	SD	Tt Diff. (Mut-WT)	Tt-T1 (min)	Tt-T2 (min)	Tt-T3 (min)	Mean	SD	Tt Diff. (Mut-WT)
Wild	17.02	17.1	14.09	16.1	1.7	NA	22.1	22.9	20.0	21.7	1.5	NA	-5.6
F1-Mu-A	15.51	15.28	13.87	14.9	0.9	-1.2	26.5	33.8	25.7	28.6	4.5	6.9	-13.7
F1-Mu-G	17.22	16.4	14.86	16.2	1.2	0.1	33.5	37.1	34.7	35.1	1.8	13.4	-18.9
F1-Mu-C	16.82	17.43	14.61	16.3	1.5	0.2	31.8	33.5	31.3	32.2	1.1	10.5	-15.9
F2-Mu-A	21.1	21.73	18.14	20.3	1.9	4.3	36.4	38.9	30.3	35.2	4.4	13.5	-14.9
F2-Mu-T	20.64	22.52	18.56	20.6	2.0	4.5	32.8	41.1	34.3	36.1	4.4	14.4	-15.5
F2-Mu-G	20.73	20.82	19.78	20.4	0.6	4.4	44.9	36.7	35.3	39.0	5.2	17.3	-18.5
F3-Mu-A	17.55	15.93	15.21	16.2	1.2	0.2	27.9	25.3	21.0	24.7	3.5	3.0	-8.5
F3-Mu-G	16.93	17.57	16.35	17.0	0.6	0.9	23.0	23.7	21.7	22.8	1.0	1.1	-5.9
F3-Mu-C	18.09	16.66	15.44	16.7	1.3	0.7	25.4	28.1	22.1	25.2	3.0	3.5	-8.4
BLP-Mu-A	23.45	21.55	19.09	21.4	2.2	5.3	46.7	45.1	35.0	42.2	6.3	20.6	-20.9
BLP-Mu-T	21.81	24.04	19.48	21.8	2.3	5.7	46.8	49.7	36.6	44.3	6.9	22.6	-22.6
BLP-Mu-G	21.97	24.18	21.43	22.5	1.5	6.5	37.6	40.3	39.1	39.0	1.3	17.3	-16.5

SD, standard deviation; NA, not applicable; Diff., difference; Mut, mutants; WT, wild type; Tt, time threshold; T1-T3, replicates 1–3; Conv., conventional RT-LAMP method; Novel, the mismatch-tolerant RT-LAMP method; min, minute. The Tt differences are highlighted in bold.