Figure 8.

dMP administration in recent-onset NOD mice reverses T1D for a limited time period. Newly diabetic NOD mice (≥240 mg/dL on consecutive days) were serially enrolled in a recent-onset diabetes reversal study. Upon enrollment, animals received a sustained release insulin pellet to temporarily (∼2 weeks) control glycemia and immediately began MP treatment (left: study timeline). Mice received the dMP (n = 11), a soluble bolus of the four factors plus unloaded MPs (n = 8), or no treatment (n = 10) (right). Animals received MP injections (arrows) three times in the first week and three weekly booster injections. Blood glucose levels were monitored twice weekly, and mice were removed from the study upon diabetes recurrence. Survival data are fit using the Kaplan–Meier nonparametric survival analysis model, and statistical analysis was performed via log-rank test (Mantel–Cox method).