Fig. 4.

Effects of twice-daily administration of (DAla²)GIP and (DAla²)GIP/xenin-8-Gln on insulin sensitivity and pancreatic insulin content in high-fat-fed mice. Insulin (15 U/kg) was given by i.p. injection (t = 0) in non-fasted mice following 21 days treatment with saline vehicle, (DAla²)GIP or (DAla²)GIP/xenin-8-Gln hybrid (each at 25 nmol/kg). Blood glucose was measured (a), area above the curve (AAC) was calculated using identical baseline subtraction for each group (b) and pancreatic insulin content was measured by RIA (c). White squares and light-grey bars, saline control; white circles and dark-grey bars, (DAla²)GIP; black circles and black bars, (DAla²)GIP/xenin-8-Gln; black triangle and white bars, lean control. Values represent means ± SEM for 6–8 mice. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with high-fat controls; ^† p < 0.05 compared with lean controls