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Analysis of 3-O-sulfation oligosaccharides by sHS. [35]S-sulfate products were analyzed using a DEAE-Sepharose column and the 35S radioactivity was measured by liquid scintillation counting. (A) The oligosaccharide Glc-GlcNAc was used as a substrate for the reaction. (B) The oligosaccharide IdoA2S-GlcNS was the acceptor for the sHS activity reaction. The blank run value was subtracted from each test compound.
