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Microscope observations during differential lysis procedure. (a) Spermatozoa heads and detached tails after incubation in Digestion buffer. (b) Washed sperm heads in Digestion buffer without Dithiothreitol. (c) Unwashed control: sperm tails after incubation with Digestion buffer containing Dithiothreitol and Proteinase K. Scale bar: 25 µm.
