Table 1.
List of primers
| Target | Primer name | Primer sequence (5′→3′) | Amplicon length |
|---|---|---|---|
| LCR | HPV31_7577_F | TTTCCTGCCTAACACACCTTGCC | 230 bp |
| HPV31_7806_R | TGCATGACACAACCTTGGCAGTT | ||
| LCR | HPV44_7604_F | GCACAGCTAGGCGCGGTTTGG | 195 bp |
| HPV44_7798_R | GATACCGGTCGCAGGTGTGTGA | ||
| LCR | HPV73_7245_F | TGGTTTACTTGACTACTGTGCTTCCATT | 216 bp |
| HPV73_7460_R | TAACTGCCAGGTGGCGCCTT | ||
| LCR | HPV66_7512_F | TTCGGTTGCCTAGCCTTTTGTCC | 159 bp |
| HPV66_7670_R | GCCCAACAGCGCCAAAGTATGC | ||
| LCR | HPV89_7641_F | GGTTGTGGCGCCATGCCACTT | 236 bp |
| HPV89_7876_R | TGCGTGGGCAAGTTTTGAAGCCA | ||
| LCR | HPV18_7349_F | GTGGTATGGGTGTTGCTTGTTG | 171 bp |
| HPV18_7519_R | GTGCCAGCGTACTGTATTGTGC | ||
| E1n | HPV18_912_F | CAATGGCTGATCCAGAAGGTACAG | 143 bp |
| HPV18_1054_R | CATATCCGACCCTGTGTCTGTTG | ||
| E1c | HPV18_2266_F | ATTCCTGCGATACCAACAAATAGAG | 178 bp |
| HPV18_2442_R | TTCACAAATGATATTACTGCTCCTTG | ||
| E2 | HPV18_3540_F | CAGACGTCGGCTGCTACACG | 88 bp |
| HPV18_3627_R | TAGCTGCACCGAGAAGTGGG | ||
| L2 | HPV18_4243_F | TATGGTATCCCACCGTGCCG | 126 bp |
| HPV18_4368_R | TCTGCTAACGTGGTGCCCTC | ||
| L1 | HPV18_6905_F | GGATGCTGCACCGGCTGAAAA | 172 bp |
| HPV18_7076_F | AGCAGAACGTTTGCGAGGGC |
Target genes, primer names (numbers designate the position of the 5′ end with respect to the GenBank reference sequences (AY262282 for HPV18 sequence)) and primer sequences used in qPCR experiments. Sizes of the amplicons are also indicated.