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. 2019 May 14;38:191. doi: 10.1186/s13046-019-1191-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — siRNA-mediated SGK3 downregulation inhibits PDAC cells growth. AsPC-1 (a), CFPAC-1 (b) and HPAF-II (c) cells were transfected with two siRNAs specifically targeting SGK3 or a non-targeting siRNA (siControl) and then manually counted with trypan blue exclusion as specified in the Methods. Data are expressed as percentage of cells transfected with siControl and are means ± SEM of n ≥ 3 independent experiments performed in duplicate. T-test and GraphPad Prism version 6.0 were used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001. (d) Representative blot confirming efficient SGK3 downregulation in all cell lines. GAPDH was used as loading control