Table 2.
Sequences and accession numbers of oligonucleotide primers used for real-time PCR and the length of the PCR products
| Gene name | Oligonucleotide sequences (5’to3’) of primers | GenBank accession number | Product length, bp |
|---|---|---|---|
| GAPDH | F: CCACGTTGTAGCCGACATC | NM001034034 | 201 |
| R: CCCTGAAGAGGACCTGTGAG | |||
| β-actin | F: CACCGCAAATGCTTCTAGGC | NM_173979.3 | 186 |
| R: TGTCACCTTCACCGTTCCAG | |||
| HPRT | F: GACCAGTCAACAGGCGACAT | NM_001034035.2 | 130 |
| R: TGACCAAGGCAAGCAAAGTC | |||
| INSR | F: AGGAGCTGGAGGAGTCCTCGTTCA | XM005208817.2 | 147 |
| R: CATTCCCCACGTCACCAAGGGCTC | |||
| GLUT4 | F: TTCATTGGCGCCTACTCAGG | NM174604.1 | 142 |
| R: CTAGCACCTGGGCGATTAGG | |||
| TNFα | F: CCACGTTGTAGCCGACATC | NM173966 | 155 |
| R: CCCTGAAGAGGACCTGTGAG | |||
| PPARγ | F: ACTTTGGGATCAGCTCCGTG | NM181024.2 | 137 |
| R: GTCAGCTCTTGGGAACGGAA |
GAPDH Glyceraldehydes 3-phosphate dehydrogenase, HPRT Hypoxanthine phosphoribosyl-transferase, INSR insulin receptor, GLUT4 Glucose transporter 4, TNFα Tumor necrosis factor-alpha, PPARγ Peroxisome proliferator-activated receptor gamma