Table 3.
Patient-derived pediatric glioma tumor and normal brain tissue specimens analyzed
| TNC score | p-value | |||||
|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | |||
| WHO-Grade (n = 50) | I | 2 | 7 | 1 | 2 | *0.05 |
| II | 1 | 1 | 3 | 2 | ||
| III | 1 | 1 | 2 | 6 | ||
| IV | 1 | 4 | 5 | 11 | ||
| Recurrence (n = 13) | Yes | 2 | 0 | 0 | 4 | 0.07 |
| No | 0 | 2 | 2 | 3 | ||
| Progression free survival (n = 33) | 15.2 | 15.2 | 19.9 | 5.7 | *0.0179 | |
| K27 M Status (n = 50) | MT | 1 | 11 | 4 | 13 | *0.012 |
| WT | 4 | 2 | 7 | 8 | ||
| Notch3 (n = 39) | Present | 2 | 8 | 4 | 13 | *0.029 |
| Abscent | 1 | 2 | 7 | 2 | ||
| Notch1 (n = 48) | Present | 4 | 8 | 8 | 15 | 0.57 |
| Abscent | 1 | 5 | 3 | 4 | ||
| PDGFRa (n = 42) | 1 | 0 | 2 | 4 | 3 | 0.68 |
| 2 | 1 | 5 | 5 | 6 | ||
| 3 | 2 | 5 | 2 | 7 | ||
Descriptive statistical analysis of immunohistochemical (IHC) staining results. TNC expression was found to directly correlate with tumor histologic grade, with highest TNC expression in high-grade tumors (WHO III and IV, p = 0.05). TNC expression also directly correlated with clinical tumor recurrence (p = 0.07), and inversely correlated to progression free survival (p = 0.018). TNC over-expression was more common in H3K27 M mutant tumors (p = 0.012), and was associated with NOTCH3 immunopositivity (p = 0.012). Between groups comparison was performed using Fischer’s exact test. MT = H3K27 M positive, WT = H3K27 M negative. * = p < 0.05