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. 2019 May 14;20:377. doi: 10.1186/s12864-019-5758-2

Table 1.

Summary of the transcriptome data from PacBio RSII platform and Illumina Hiseq platform

Platform
Illumina Hiseq (n = 15)
 Raw reads (average) 5,562,602.5
 Clean reads (average) 53,312,258.5
 Clean bases (G, total) 119.97
 Base error rate (average) 0.015%
 Q30 (average) 93.46%
 GC content (average) 47.05%
 Unigenes annotated in at least one database (NR, NT, KO, Swissprot, Pfam, GO, KOG) 129,325
 Unigenes annotated in all databeses 29,191
 Total unigenes 130,553
 TF number 8006 (6.19%)
PacBio RSII (n = 15)
 Total CCS 617,063
 Full length reads 467,672 (75.9%)
 FLNC reads 434,967 (70.5%)
 Average FLNC read length 1720 bp
 Consensus reads 199,106
 Subreads base (G) 13.46
 Subreads number 14,267,901
 Average subreads length 944 bp
 N50 1572
 Total unigenes annotated in at least one database (NR, NT, KOG, Swissprot, Pfam, GO, KEGG) 140,196
 Total unigenes 44,938
 TF number 9407 (5.08%)

CCS circular consensus sequences, FLNC full-length non chimera, GO Gene Ontology, KEGG Kyoto Encyclopedia of Genes and Genomes, KO KEGG Ortholog database, KOG euKaryotic Orthologous Groups, Nr NCBI non-redundant protein sequences, Nt NCBI non-redundant nucleotide sequences, Pfam Protein family, TF transcription factors