Fig. 2.

SNHG12-knockdown inhibited malignant progression in renal cancer cells. a Caki-1 and ACHN cells were transfected with either scramble or SNHG12 shRNA using lipofectamine 2000. The knockdown was measured by real-time PCR at 24, 48 and 72 h post-transfection. ****p < 0.0001; b, c Cell viability was determined in SNHG12-deficient ACHN and Caki-1 cells with CCK-8 kit. d The anchorage-independent growth of both SNHG12-proficient and -deficient ACHN and Caki-1 cells was interrogated by soft agar assay in triplicate, the statistical results from three individual fields were shown in pane E, ****p < 0.0001. f, g The apoptotic cells were analyzed with Annexin-V/7-AAD double-staining method, followed by flow cytometry semi-quantitation. ****p < 0.0001. h Cleaved PARP and Caspase 3 in response to SNHG12 silencing were determined by western blotting. β-Actin was used for loading control