Fig. 1.

High glucose reduces NK cells^, killing effect on pancreatic cancer cells through suppressing cell surface MICA/B expression. The PANC-1 cells and SW1990 cells were treated with different concentrations of glucose (5 mM, 15 mM and 25 mM) for 24 h respectively. a The killing effects of NK cells on pancreatic cancer cells were detected by LDH release assay. E: T = Effector cells: target cells. b-c The cell surface expression of MICA/B was detected by qRT-PCR (b) and Western blot (c). d MFI (folds) of MICA/B detected by flow ctyometry was evaluated with a Student t test from three independent experiments. e Representative histograms of flow cytometry demonstrating MICA/B expression in pancreatic cell treated with different concentrations of glucose. f The killing effects of NK cells on pancreatic cancer cells in the presence of anti-MICA/B antibody treatment (displayed as mean value). The graphs shown were from three independently experiments. The data were from three independently repeated experiments. **P < 0.01; *P < 0.05