Fig. 3.

High glucose reduces the cell surface expression of MICA/B by promoting Bmi1 expression. a-c Expression of Bmi1 detected by qRT-PCR (a) Western blot (b) and immunofluorescence (c) in two pancreatic cancer cell lines treated with high glucose for 24 h. d-e Expression of Bmi1 detected by qRT-PCR (d) and Western blot (e) after transfection with Bmi1-siRNA under high glucose environment. f-g Cell surface expression of MICA/B at mRNA and protein levels detected by qRT-PCR (f) and Western blot (g) in both pancreatic cancer cell lines after Bmi1 knockdown under high glucose environment. h The killing effect of NK cells on pancreatic cancer cells after Bmi1 knockdown under high glucose environment. The graphs show representative results from three independently repeated experiments. The data were from three independently repeated experiments. Scale bar, 50 μm, **P < 0.01