Fig. 4.

High glucose promotes the expression of GATA2, which inhibits cell surface expression of MICA/B. a-c The cell surface expression of MICA/B were measured by Western blot (b) and qRT-PCR (c) after transfected with GATA2 siRNA (a). d Representative histograms of flow cytometry demonstrating MICA/B expression in pancreatic cells transfected with GATA2 siRNA. MFI (folds) of MICA/B was evaluated with a Student t test from three independent experiments. e-f The expression of GATA2 were detected by qRT-PCR (e) and Western blot (f) in pancreatic cancer cells treated with high glucose. g-i The expression of MICA/B were detected by qRT-PCR (H) and Western blot (i) after GATA2 siRNA transfection (g, i) under high glucose environment. j The potential site of MICA/B matching the binding sequence of GATA2 is shown in upper panel. The low panel showed that high glucose treatment promoted the binding of GATA2 to the promoters of MICA/B. The graphs shown were representative results of three independent experiments. **P < 0.01