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. 2019 May 14;19:129. doi: 10.1186/s12935-019-0845-7

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Western blot for the detection of stemness-associated markers. a Cells were pretreated with or without 2 Gy of IR and were analyzed for the expression of cleaved caspase 3, cleaved caspase 9, caspase 3, caspase 9, Bax, and Bcl2 protein levels through Western blotting. b Knockdown of PKM2 combined with IR downregulated NANOG/OCT4/SOX2 in protein expression. GAPDH protein was used as an internal standard. Data represent three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. (shPKM2: shRNA against PKM2; IR: irradiation. 1 = control cells, 2 = control cells + IR, 3 = shPKM2 cells, 4 = shPKM2 cells + IR)