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. 2019 May 8;10:502. doi: 10.3389/fphys.2019.00502

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Copyright © 2019 Kha, Guerin and Tseng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Regrown eyes regain Pax6 patterning by 3 dps. Images shown are immunostained, transverse sections at three developmental timepoints corresponding to 1, 2, and 3 days post surgery (dps). (A,B) Pax6 expression in the regrowing eye is less organized at 1 dps but regains patterning similar to contralateral control eyes (unoperated) by 3 dps. White dashed lines delineate each regrowing eye. (A’,B’) Images shown in panels A’ and B’ correspond to the region shown in the inset box in panel A4 for the corresponding A or B panel at high magnification. Blue color indicates nuclear staining (TO-PRO-3). Green color indicates anti-Pax6 signal. Sample sizes: 1 day, n = 5; 2 days, n = 7; and 3 days, n = 6. (A,B, A’,B’) Up = dorsal, down = ventral, lens is on the left. Scale bar: A,B = 100 μm and A’,B’ = 50 μm.