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. 2019 May 7;27(6):1920–1933.e7. doi: 10.1016/j.celrep.2019.04.042

Figure 2.

Figure 2

Downregulation of Multiple Collagens, Protocadherins, and Innate Immune Mediators

(A) Functional enrichment within all proteins that were downregulated >2-fold at any point during infection compared to 18-h mock samples. A background of all quantified human proteins was used. Shown are representative terms from each cluster with Benjamini-Hochberg-corrected p values of < 0.05. Components of each significantly enriched cluster are shown in Table S2E. A similar analysis was performed for proteins upregulated >2-fold; however, this did not reveal any significantly enriched clusters.

(B) Example temporal profiles of collagens, innate immune mediators, and protocadherins. Data are represented as mean ± SEM (n = 3); ∗p < 0.05 and ∗∗p < 0.01 (see STAR Methods). Error bars and statistics are not included on the plots for PCDHGA6, PCDHGB4, and PCDHB8, as these proteins were not quantified in all three replicates; full data are shown in Table S1.

(C) Validation of temporal profiles shown in (B) by immunoblot of HFFFs infected with VACV (MOI = 5). Viral proteins D8 and C6 were representative of late and early gene expression, respectively.