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. 2018 Dec 10;108(3):172–189. doi: 10.1159/000496106

Fig. 1.

Fig. 1

Exposure to female urine regulates the activity of MeAKiss neurons and impacts luteinizing hormone (LH) levels in Kiss1CreEGFP/wt/Rosa26lox-stop-lox-tdTomato/wt male mice. a Co-localization of c-Fos (green) in MeAKiss neurons (tdTomato+; red) of male mice exposed to control (top panel) or female urine (bottom panel). Yellow arrows indicate tdTomato+ neurons that express c-Fos. Scale bars, 100 µm. b Number of c-Fos+ cells in the MeA subdivisions after exposure to control or urine (n = 6 in each group). c Percentage of MeAKiss (tdTomato+) neurons that express c-Fos in the MeA after control or urine exposure (n = 6 in each group). d Serum LH concentrations (ng/mL) in male mice 15 min post-exposure (n = 5 in each group). e Percentage of c-Fos+ AVPVKiss (tdTomato+) neurons after exposure to control or urine (n = 4 in each group). f Percentage of c-Fos+ ARCKiss (tdTomato+) neurons after exposure to control or urine (n = 3 in each group). Data are represented as mean ± SEM. Values of individual mice are plotted as circles. ns, no significant difference; * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student's t test). opt, optic tract, MeAa, anterior medial amygdaloid nucleus, MeApv, posteroventral medial amygdaloid nucleus, MeApd, posterodorsal medial amygdaloid nucleus.