Fig. 2.

Verification and comparison of MeA neuron targeting using Kiss1^CreEGFP/wt/Rosa26^{lox-stop-lox-tdTomato/wt} mice and Cre-dependent rAAV infection of Kiss1^CreEGFP/wt mice. a Representative photomicrograph showing tdTomato expression (red) and Kiss1 mRNA expression (white) in the MeA of a double transgenic Kiss1^CreEGFP/wt/Rosa26^{lox-stop-lox-tdTomato/wt} male mouse. White arrows mark tdTomato⁺ cells that are positive for Kiss1 expression. b Representative photomicrograph showing tdTomato expression (red) and negligible RNAscope in situ signal (white) with a negative control probe (dapB; a bacterial gene) in the MeA of a double transgenic Kiss1^CreEGFP/wt/Rosa26^{lox-stop-lox-tdTomato/wt} male mouse. c Representative photomicrograph showing mCitrine (green) labelling in the MeA (Bregma 1.34 mm) of male Kiss1^CreEGFP/wt mice infected with rAAVs encoding hSyn-DIO-hKORD-IRES-mCitrine. d Representative photomicrograph of the MeA (Bregma 1.34 mm) labelled with tdTomato from a double transgenic Kiss1^CreEGFP/wt/Rosa26^{lox-stop-lox-tdTomato/wt} male mouse. e Comparison between the number of mCitrine⁺ neurons in the MeA of Kiss1^CreEGFP/wt male mice infected with AAV-hSyn-DIO-hKORD-IRES-mCitrine and tdTomato⁺ neurons in the Kiss1^CreEGFP/wt/Rosa26^{lox-stop-lox-tdTomato/wt} male mice. f Distribution of fluorescently labelled neurons in the MeA between 1.34 and 1.94 mm posterior to Bregma in Kiss1^CreEGFP/wt male mice infected with AAV-hSyn-DIO-hKORD-IRES-mCitrine (virally induced expression) and Kiss1^CreEGFP/wt/Rosa26^{lox-stop-lox-tdTomato/wt} male mice (transgenic induced expression). Scale bars, 100 µm.