Fig. 5.

Chemogenetic activation of MeA^Kiss neurons reduces neuronal activity in the POA in male mice. Male Kiss1^CreEGFP/wt mice received MeA injections of rAAVs encoding EF1α-DIO-hM3D(Gq)-mCherry and hSyn-DIO-hKORD-IRES-mCitrine. After 3 weeks, the mice were treated with SALB (10 µg/g), CNO (3 µg/g), or vehicle (DMSO). a Representative photomicrographs showing Hoechst-stained nuclei (magenta) and c-Fos⁺ nuclei (yellow) in the POA (Bregma −0.10 mm) 60 min after treatments. Arrowheads mark c-Fos⁺ nuclei. Scale bars, 100 µm. b Number of c-Fos⁺ nuclei in the POA analysed 60 min after treatments (n = 3 in each group). Data are represented as mean ± SEM. Circles represent the individual values for each mouse. ns, no significant difference; * p < 0.05, (one-way ANOVA followed by Tukey's multiple comparison test).