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. 2018 Nov 22;104(5):973–985. doi: 10.3324/haematol.2018.199661

Figure 1.

Figure 1.

CD147 is down-regulated during monocytic (Mo) and granulocytic (G) differentiation of CD34+ hematopoietic progenitor cells (HPCs) and is involved in HPC proliferation. (A) qRT-PCR analysis of CD147 mRNA expression during selective Mo and G proliferation and differentiation of CD34+ HPCs, as compared to U937 and HL-60 leukemic cells. (B) Western blot analysis of CD147 protein expression level in CD34+ cells and Mo and G differentiating HPCs; Jurkat and SKBR3 cells are shown as positive controls of CD147 expression; HG- and LG- are indicated for High- and Low- glycosylated CD147 isoforms; actin is shown as an internal control; molecular weights are indicated (kDa). (C) Flow cytometry analysis of CD147 membrane protein expression during Mo and G differentiation and maturation of HPCs. (D) Flow cytometry analysis of CD147 membrane protein expression in Mo and G differentiating transfected (CD147-siRNA)-HPCs, as compared to transfected (c-siRNA)-HPCs of control, at day 2 of Mo and G cultures corresponding to day 3 post transfection. (E) Cell growth inhibition of (CD147-siRNA)-HPCs, as compared to (c-siRNA)-HPCs, grown under both G and Mo liquid culture conditions. (F) Clonogenic assays performed under G and Mo culture conditions with (CD147-siRNA)-HPCs, as compared to (c-siRNA)-HPCs. (A, C-F) Mean±Standard Error of Mean of three independent experiments is shown. *P<0.05; **P<0.01; ***P<0.001. (B) One representative experiment out of three is shown. AU: arbitrary units; MFI: mean fluorescence intensity.