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. 2018 Nov 22;104(5):973–985. doi: 10.3324/haematol.2018.199661

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Figure 6. — AC-73 decreases the survival of CD147⁺ acute myeloid leukemia (AML) cells exposed to chemotherapeutic agents and activates autophagy in AML cells. (A) CD147 is expressed in the subfraction of CD34⁺CD371⁺ positive AML leukemic cells. (B) Dose response analysis of AC-73 treatment on leukemic cell apoptosis of AML-M2, AML-M3 and AML M5 blasts obtained from 5 AML (4, 5, 7, 8 and 10) patients, as compared to respective control (C) leukemic blasts; flow cytometry analysis of total apoptosis by Annexin V/PI (%) is shown. (C) Western blot analysis of the autophagic-related LC3 marker shows its conversion from the LC3-I to LC3-II form in leukemic blasts treated in vitro with growing concentration of AC-73 as compared to control leukemic blasts (−). (D) Cell viability assays were performed on M2, M3 and M5 leukemic blasts, obtained from 5 AML patients (4), (5), (7), (8) and (10), and treated in vitro by AC-73 (2.5 μM) in combination with ATO or Ara-C (0.1 and 1 μM), as compared to control (C) leukemic blasts and to single treatment with AC-73, ATO and Ara-C. (A) One representative experiment is shown. (B and D) Mean±Standard Error of Mean of three independent experiments is shown. *P<0.05; **P<0.01; ***P<0.001; ns: not significant. (C) One representative western blot experiment out of three is shown. Actin was used as an internal control.