Figure 2.

Adhesion of red blood cells to endothelium activated or not by tumor necrosis factor-α. (A) Adhesion of healthy (AA) or sickle cell disease (SCD) red blood cells (RBC) on immortalized endothelium (EA926.hy) treated or not with tumor necrosis factor-α (TNF-α) under flow conditions (data are expressed as cells/mm²). The data were obtained from six separate comparable experiments. All calculations were performed using the IBM SPSS 20.0 statistical package (IBM Inc., Armonk, NY, USA). The results of the adhesion tests are expressed as median values with the minimum-maximum range and are illustrated by box plots. The data were analyzed with non-parametric tests, the Mann-Whitney U test for unpaired samples and the Wilcoxon signed-rank test for paired samples. A value of P<0.05 is considered statistically significant. (B) Dose-response curve for factor H (FH) in adhesion assays for healthy (AA) or sickle (SCD) RBC. Data were obtained at 6 min flux on endothelium treated with either vehicle or TNF-α. The curves are representative of six separate and independent experiments with similar results.