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. 2019 May 8;7:78. doi: 10.3389/fcell.2019.00078

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Copyright © 2019 Lin and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphatidic acid (PA) is enriched at macropinosome but not lysosome upon OS treatment. (A,B) The enrichment and duration of PA at macropinosome. PABD-GFP expressing myotube was subjected to OS treatment and time-lapse microscopy images were shown. The intensity of PABD-GFP at the macropinosome or cytosol were quantified upon OS treatment and shown in (B). Eight macropinosomes in (A) were quantified and analyzed. (C) PABD-GFP was not accumulated on lysosome upon OS treatment. After incubation with lysotracker for 15 min, PABD-GFP expressing myotube was imaged with time-lapse microscopy upon OS treatment. Boxed areas were magnified and shown. Scale bars: 10 mm. ^∗p < 0.05.