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. 2019 Jan 16;247(3):293–304. doi: 10.1002/path.5179

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© 2018 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PATH-5179-FIG-0003-c — Polarised ABCB1 functions as an orientation‐dependent drug effluxer. (A) C3953 colonies cultured in either Matrigel/serum or as serum‐free suspensions for 1 week and labelled with the fluorescent ABCB1 substrate R123 (green) for 1 h. The same colonies were then fixed and labelled for F‐actin (red) with DAPI (blue). Merge shows R123/DAPI/phase contrast/F‐actin. (B) R123 fluorescence of C3953 colonies grown in Matrigel/serum for 1 week in the presence of either DMSO (vehicle) or 50 μm verapamil. (C) R123 labelling of live Matrigel/serum‐grown lumen‐forming colorectal cell lines (C80, SW1222, OXCO2) and non‐lumen‐forming cell lines (HCT116, HT29 and DLD1). (D) Doxorubicin auto‐fluorescence of live C105251 colonies cultured in either Matrigel/serum for 1 week or as serum‐free suspension cultures and incubated with 10 μm doxorubicin for 1 day. Scale bars = 50 μm.