Figure 4.

Integrin α2β1 mediates the interaction of primary cultures with collagen. (A) Microarray gene expression analysis of various integrin subunits in the primary cultures. (B) Phase‐contrast images of collagen I gels in which C105251 primary cultures were incubated with various inhibitors or controls for 3 days. These include isotype control mouse monoclonal IgG, AIIB2 (rat monoclonal against integrin β1 subunit), BIIG (control rat monoclonal against integrin α5 subunit), P5D2 (mouse monoclonal against integrin β1 subunit), BHA2.1 (mouse monoclonal against α2β1 integrin heterodimer). Each antibody was used at 10 μg/ml. Other inhibitors tested included obtustatin (α1β1 inhibitor, 5 nm), blebbistatin (myosin II inhibitor, 100 μm). (C) Graph showing quantification of C105251‐mediated gel contraction, of the same experiment as (B) with the additional inhibitors nocodazole and cytochalasin B (microtubule and actin inhibitors, respectively, 1 μm each). DMSO was the vehicle control. *p < 0.05 versus appropriate control, Dunnett's multiple comparisons test. Error bar = standard deviation.