Figure 4.

Adoptive transfer of donor nT‐reg inhibits recipient autoantibody responses and prolongs allograft survival. C57BL/6 (B6) recipients of bm12 heart allografts were adoptively transferred the day after transplantation with natural T‐regs (nT‐regs) purified from a donor (bm12) or recipient (B6) strain, and recipient autoantibody responses (A), allograft survival (B), and allograft vasculopathy (C) were assessed as detailed in Figure 2 legend. Control recipients received no treatment. Whereas administration of recipient‐strain nT‐regs had little impact on rejection responses or rejection kinetics, administration of donor‐strain nT‐regs inhibited effector autoantibody responses (*P = .27, **P < .001, 2‐way ANOVA), prolonged allograft survival (MST 91 vs 67 days; *P = .03. log‐rank test) and was associated with reduction in the severity of allograft vasculopathy (‡P = .02, ‡‡P = .38; Mann‐Whitney test). Data are representative of 6 animals per group, and expressed as mean ± SD, n = 6