Figure 6.

CMAS gene expression levels are correlated to recurrence‐free survival in human colorectal cancer and a partial CMAS knock out is sufficient to drive tumor growth in vivo. (a) CMAS gene expression data was analyzed in the GSE39582 colorectal cancer cohort.27 Patients were stratified in two groups with either low CMAS gene expression (median < 150) or high CMAS gene expression (median > 300) and evaluated for their recurrence‐free survival. (b) MC38‐MOCK, MC38‐Sia^null or MC38‐MOCK cells (30%) mixed with MC38‐Sia^null cells (70%) were injected into C57Bl/6 mice (n = 9) and sacrificed when the tumor reached a size of 2000 mm³. Tumor growth was measured and survival curves of the mice are displayed. (c) Flow cytometric analysis of viable CD8⁺ T cells (CD3⁺CD8b⁺) at the tumor site when the tumor reached a size of 2000 mm³. Mean ± SD; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. [Color figure can be viewed at wileyonlinelibrary.com]