Figure 2.

Addition of recognition elements to the scaffold reduces the current observed in the absence of target in a size-dependent manner. (a) To show this we attached either the 7 kDa P3 domain of CheA or the full length, 71 kDa CheA to the scaffold. (b) Upon the addition of the P3 domain the signaling current is reduced by only ~15% relative to that seen for the unmodified scaffold (the dark bars represent the signal change seen for control sensors lacking NTA). Addition of the full-length protein, in contrast, causes the signaling current to decrease by 35%. Throughout, the error bars reflect the standard deviations of replicates performed using multiple independently fabricated sensors.