. Author manuscript; available in PMC: 2019 May 15.

Published in final edited form as: Metabolomics. 2017 Dec 23;14(1):15. doi: 10.1007/s11306-017-1310-z

Table 1.

List of metabolites analysed by this approach, retention times, the MS/MS transitions used for quantification and the collision energy voltages

Analyte	Retention time (min)	Polarity	Transitions (m/z)	Collision Energy (V)

NAM	2.36	+	123 > 80	25
²H₄-NAM	2.36	+	127 > 80	25
Adenosine	2.87	‑	266 > 134	25
¹³C₅-Adenosine	2.87	‑	271>134	25
MeNAM	4.48	+	137 > 80	20
NA	5.97	+	124 > 78	25
cAMP	6.09	‑	328>79, 134	25
¹³C₅-cyclic AMP	6.10	‑	333 > 134	25
NMN	6.63	+	335 > 123	20
NAD	6.65	+	664>428, 524	18
NAAD	10.69	+	665>136, 428	35
NADH	10.70	+	666 > 514, 649	20
AMP	10.71	‑	346 > 79, 97, 134	25
¹³C₅-AMP	10.73	‑	351>134	25
cADPR	10.74	‑	540>273, 408	30
ADPR	10.74	‑	558>346	28
NAMN	10.78	+	336 > 97.6, 124	25
NADP+	10.79	+	744>136, 508	30
ADP	11.19	‑	426 > 134, 159	25
¹⁵N₅-ADP	11.20	‑	431>139, 159	25
NADPH	13.28	+	746 > 729	20
ATP	16.54	‑	506>159, 408	25
¹³C₁₀¹⁵N₅-ATP	16.60	‑	521>159, 408	25