Table 2.
The r2 and limit of quantification (LOQ: signal to noise ratio > 10) of each analyte quantified using this LC/MS/ MS assay
| Analyte | Internal Standard Used | LOQ (pmol on column) |
r2 (Stds in buffer) |
aMatrix effect (%) |
|---|---|---|---|---|
| NAM | 2H4-NAM | 0.2 | 0.9909 | + 10 |
| Adenosine | 13C5-ADO | 0.05 | 0.9934 | 0 |
| MeNAM | 2H4-NAM | 0.01 | 0.9918 | –20 |
| NA | 2H4-NAM | 2.0 | 0.9949 | +17 |
| cAMP | 13C5-Cyclic AMP | 0.05 | 0.9985 | –4 |
| NMN | 13C5-Cyclic AMP | 0.1 | 0.9894 | –4 |
| NAD+ | 13C5-Cyclic AMP | 0.1 | 0.9913 | –3 |
| NAAD | 2H4-NAM | 0.02 | 0.9882 | –18 |
| NADH | 13C1015N5-ATP | 0.2 | 0.9878 | –17 |
| AMP | 13C5-AMP | 0.2 | 0.9912 | –4 |
| cADPR | 2H4-AMP | 0.2 | 0.9929 | 0 |
| ADPR | 2H4-AMP | 0.2 | 0.9911 | 0 |
| NAMN | 2H4-AMP | 0.2 | 0.9821 | –20 |
| NADP+ | 15N5-ADP | 0.04 | 0.9901 | +7 |
| ADP | 15N5-ADP | 0.4 | 0.9958 | +6 |
| NADPH | 2H4-NAM | 0.2 | 0.9923 | –7 |
| ATP | 13C1015N5-ATP | 0.4 | 0.9929 | –5 |
The standard curves are shown in the supplementary file 1
a
positive values denote signal enhancement and negative values indicate signal suppression