Continuous, calibration-free measurement of cocaine and doxorubicin in flowing whole blood. (A) When challenged in vitro in flowing, undiluted whole blood the absolute peak currents of cocaine-detecting E-AB sensors vary significantly from sensor to sensor and even for a single sensor over several hours (compare the zero-target baselines of individual sensors at 1 and 6 h). (B) Dual-frequency calibration-free sensing nevertheless produces reasonably accurate concentration estimations of cocaine (spiked concentrations shown as a gray dashed line) even under these demanding conditions. (C) Doxorubicin-detecting sensors exhibit similar variability when placed in flowing whole blood. (D) Dual-frequency calibration-free measurements, however, once again suppress this variability, producing concentration estimates in close agreement with the spiked concentration (gray dashed line).