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. 2019 May 8;10:1016. doi: 10.3389/fimmu.2019.01016

Figure 1.

Figure 1

Hematopoietic-specific deletion of Foxo1 resulted in increased proportion and quantity of CLP, pre-pro NK, and NK cells. (A) Intracellular flow cytometric analysis of Foxo1 expression (mean fluorescence intensity (MFI) relative to littermate control) in splenic NK cells (CD3−CD19−NKp46+) from control vs. Foxo1Δ/Δ mice. (B) Frequency and enumeration of LSK (Lin−CD127−Sca-1+c-Kit+) and CLP (Lin−CD127+Sca-1+c-Kit+) in the bone marrow (BM) from control vs. Foxo1Δ/Δ mice. Lin: CD3, CD19, B220, CD4, CD8, CD11b, NKp46, Gr1, and Ter119. (C) Frequency and enumeration of pre-pro NK a (Lin−Sca-1+c-KitintCD135−CD127+) and pre-pro NK b (Lin−Sca-1+c-Kit−CD135−CD127+) in the BM from control vs. Foxo1Δ/Δ mice. Lin: CD3, CD19, B220, Gr1, Ter119, CD4, CD8, CD11b, and NKp46. (D) Frequency and enumeration of Lin−CD122+ cell in the BM from control vs. Foxo1Δ/Δ mice. Lin: CD3, CD19, Gr1, and Ter119. (E) Frequency and enumeration of NK cells (CD3−CD19−NKp46+) in the BM from control vs. Foxo1Δ/Δ mice. Ratio of LSK, CLP, pre-pro NK a and pre-pro NK b cells are relative to total BM cells (A–C), whereas ratio of Lin−CD122+ and CD3−CD19−NKp46+NK cells are relative to total lymphocytes. Each dot represents one mouse. At least 3 littermates were included for (A–E) (Error bars indicate SD; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, control vs. Foxo1Δ/Δ mice; unpaired Student's t-test with generalized linear models).