Skip to main content
. 2019 May 8;10:1016. doi: 10.3389/fimmu.2019.01016

Figure 4.

Figure 4

Hematopoietic-specific deletion of Foxo1 promotes NK cell maturation. (A) Flow cytometric analysis and frequencies of indicated subsets in NK cells (CD3−CD19− NKp46+) distinguished by the expression of CD27 and CD11b in the spleen and peripheral lymphoid node (pLN) from control and Foxo1Δ/Δ mice. (B) The calculated ratio between CD27− vs. CD27+ cells among CD11b+ NK cells in both spleen and pLN from control and Foxo1Δ/Δ mice. (C) Frequencies of CD27− among CD43low NK cells (CD3−CD19−NKp46+) in the spleen and pLN from control and Foxo1Δ/Δ mice. (D) Flow cytometric analysis and frequencies of CD43+KLRG1+ NK cells (CD3−CD19−NKp46+) in the spleen and pLN from control and Foxo1Δ/Δ mice. (E) Intracellular flow cytometric analysis of T-bet expression (MFI relative to littermate control) in NK cells (CD3−CD19−NKp46+) in the spleen from control and Foxo1Δ/Δ mice. Each dot represents one mouse. At least three littermates were included for (A–E). (Error bars indicate SD; unpaired Student's t-test with generalized linear models; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, control vs. Foxo1Δ/Δ mice).