Figure 2.

Ancient DNA recovery and human endogenous content from archaeological dental calculus. Bar charts summarizing: (a) Total DNA yield (ng/mg) in dental calculus (filled bars) and dentin samples (hollow bars) on a log 2 scale. Dental calculus samples show a higher total DNA yield compared with dentin samples in all cases. (b) Endogenous human DNA content (%) in dental calculus (filled bars) and dentin samples (hollow bars) on a log 2 scale. Overall human endogenous content is higher for dentin samples. (c) Estimated absolute human DNA yields (ng/mg) on a log 2 scale. Total human DNA per mg is estimated to be higher in dental calculus in 4 out of 12 sample pairs, but both substrates exhibit high variation. Archaeological sites are ordered from left to right by continent (Europe, Americas, Asia). Data are provided in Supporting Information Table S2