Table 1.
Main characteristics of the different data‐acquisition methods used in this study
| Data‐dependent acquisition | Data‐independent acquisition | ||||||
|---|---|---|---|---|---|---|---|
| DDA120 | DDA60 | DDA30 | DIA60 | DIA30 | DIAvw | ||
| MS1 | Resolution | 120,000 | 60,000 | 30,000 | 60,000 | 30,000 | 30,000 |
| AGC | 1.0E+06 | 1.0E+06 | 1.0E+06 | 3.0E+06 | 3.0E+06 | 3.0E+06 | |
| Fill time (ms) | 450 | 450 | 450 | 55 | 100 | 100 | |
| Scan range (m/z) | 375–1500 | 375–1500 | 375–1500 | 300–900* | 300–900 | 300–900 | |
| MS2 | Resolution | 120 000 | 60 000 | 30 000 | 30 000 | 15 000 | 15 000 |
| AGC | 1.0E+05 | 1.0E+05 | 1.0E+05 | 1.0E+06 | 1.0E+06 | 1.0E+06 | |
| Fill time (ms) | 450 | 220 | 100 | Automatic | Automatic | 115 | |
| Loop count | 10 | 10 | 10 | 10 | 10 | 85 | |
| Isolation window | 2 | 2 | 2 | 20 m/z | 20 m/z | Variable | |
| NCE | 27 | 27 | 27 | 26 | 26 | 26 | |
| Scan range (m/z) | 300–900 | 300–900 | 300–900 | ||||