TABLE 2.

Primer sequence, annealing- and melting temperature, and detected transcript variants.

Target name	Primer sequence	Annealing temp. (exp)	Melting temp. (exp)	Transcript variants
BDNF	s: TGA G AGA C G CAC AGG A	59.0°C	77.5°C	1-10 X1
NM_012513.4	as: AGA GGT A GTG TAG G GGA C
GDNF	s: G TAG G GCT C G TGA C	60.0°C	82.0°C	X1–X4
NM_019139.1	as: CCA GGG TCA GAT ACA TCC AC
L1CAM (He et al., 2012a)	s: GCCTCAGCCTCTATGTG	60.0°C	80.0°C	X1–X4
NM_017345.1	as: GCCAGTGCCATTAGTCTTC
HPRT	s: AGT CCC AGC GTC GTG ATT AG	63.5°C	78.0°C	HPRT, HPRT1
NM_012583.2	as: TGG CCT CCC ATC TCC TTC AT
Fibronectin (Yoshida et al., 2002)	s: GTGGCTGCCTTCCTTCTC	58.0°C	80.0°C	X1–X10
NM_019143.2	as: GTGGGTTGCACCTTCT
Ankrd27 (Gambarotta et al., 2014)	s: CCCAGGATCCGAGAGGTGCTGTC	62.0°C	79.0°C	X1, X6
NM_001271264.1	as: CAGAGCCATATGGACTTCAGGGGG
GFAP (Tanga et al., 2004)	s: TGG CCA CCA GTA ACA TGC	62.0°C	83.0°C	GFAP
NM_017009.2	as: CAGTTGGCGGCGATAGTCAT
TrkB (Kondo et al., 2010)	s: CACACACAGGGCTCCTTA	63.0°C	80.0°C	X1–X5
NM_012731.2	as: AGTGGTGGTCTGAGGTTGG
Itgα5	s: GTTTACACATGCCCTCTCAC	59.0°C	80.0°C	Itgα5
NM_001108118.1	as: GATTCCCTTTACAGCTCCA
Itgß1	s: A CAG T C AGA ACA GTC C	56.0°C	81.0°C	X1
NM_017022.2	as: AGG ATC G T CTC ACA ATG G
MKi-67	s: ACT GTA T T ATT GCC TGT GCT	60.0°C	78.0°C	X1
NM_001271366.1	as: CCC ACC A CAT TTA C TGC T
GFRα1	s: CTG TCT TTC TGA TAG TGA TTT CGG	56.0°C	83.5°C	X1,X2
NM_012959.1	as: GTG A CTG CTT CTC C GG
Cadm3	s: CTCTCTGTCCGACCCT	60.0°C	80.5°C	X1
NM_001047103.1	as: TTTGTGCCGGATCATAGTG
Cadm4	s: CAG TAT GAT GGG TCT ATA GTC GT	64.0°C	85.5°C	Cadm4
NM_001047107.1	as: GGA GCC ACT G ACA GTG AG
LIFR	s: CATCTACTGGGCCTTTACC	59.0°C	78.5°C	X1–X3
NM_031048.1	as: TCTCTGCTTTGTGTTGTGGA
p75	s: CTG T GCG G AGA TCC CT	60,0°C	85,0°C	X1,X2
NM_012610.2	as: G ATG GAG C TAG ACA GGA
HSP70	s: GGTGCTGATCCAGGTGTACGAGG	55.0°C	85.0°C	Hspa1a, Hspa1b, Hspa1l
NM_212546.4, NM_031971.2, NM_212504.1	as: GATGTCGGGTCACCTCGATCTGG
ß-Actin (Kondo et al., 2010)	s: CCTGTATGCCTCTGGTCGTA	55.0°C	85.5°C	Actb
NM_031144.3	as: CCATCTCTTGCTCGGTCT