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. 2019 May 8;10:497. doi: 10.3389/fphar.2019.00497

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Copyright © 2019 Zhou, Ci, Ma, Yu, Cui, Zhen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protective effect of Pts pretreatment against NaAsO₂-induced oxidative stress in HaCaT cells. The HaCaT and JB6 cells were exposed to 25 μM of NaAsO₂ for 24 h with or without Pts (3.75, 7.5, and 15 μM) pretreatment. (A,B,E) ROS generation was measured by the DCFH-DA method using a multi-detection reader and fluorescence microscope at 100× magnification. Intracellular MDA levels (C,F) and SOD (D,G) activities were determined by commercial kits. The results are expressed as the means ± S.E.M. of three independent experiments. ^##P ≤ 0.01 versus the control group, ^∗∗P ≤ 0.01 versus the NaAsO₂ group.