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. 2019 May 8;10:497. doi: 10.3389/fphar.2019.00497

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Copyright © 2019 Zhou, Ci, Ma, Yu, Cui, Zhen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protective effect of Pts pretreatment against NaAsO₂-induced apoptosis in HaCaT cells. The HaCaT cells were exposed to 25 μM of NaAsO₂ for 24 h with or without Pts (3.75, 7.5, and 15 μM) pretreatment. (A,B) The percentage of cell apoptosis was determined using flow cytometry. (C) Morphology of apoptotic cells was evaluated by fluorescence microscopy following DAPI staining at 200× magnification. Triangles: nuclear chromatin condensation in apoptotic cells. Arrows: apoptotic bodies. The results are expressed as the means ± S.E.M. of three independent experiments.^##P ≤ 0.01 versus the control group, ^∗∗P ≤ 0.01 versus the NaAsO₂ group.