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. 2019 May 8;10:497. doi: 10.3389/fphar.2019.00497

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Copyright © 2019 Zhou, Ci, Ma, Yu, Cui, Zhen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Pts on AMPK and AKT phosphorylation, Nrf2 translocation and antioxidant enzyme expression. HaCaT cells were plated in six-well culture plates and treated with Pts (3.75, 7.5, and 15 μM) for 24 h. Nuclear and cytoplasmic extracts from cells were prepared for detecting Nrf2 in panels (A,B) and total protein extracts from cells were prepared for detecting HO-1, NQO1, AMPK and AKT in panels (C–F). The results are expressed as the means ± S.E.M. of three independent experiments. ^##P ≤ 0.01 versus the control group.